Bovine trypsin proteinase

Since the third generation insulin (insulin analog) appeared, it has been favored by the majority of patients with its long-term or quick acting characteristics, and has become the fastest growing part of the market of all diabetes drugs.

The production of insulin and insulin analogues requires the cleavage of two tools: carboxypeptidase B and trypsin. However, only bovine trypsin can cut insulin analogues well, which can be used in the production of porcine trypsin, human trypsin, bovine trypsin or other trypsin. However, the bovine trypsin extracted from the pancreas of cattle, due to the existence of BSE virus, foot-and-mouth disease virus and other virus hidden dangers, the insulin analog produced and processed by the trypsin from this animal source increases the risk of virus infection of patients, and it is strictly prohibited to export to mainstream countries such as Europe and the United States. To produce more safe and effective insulin analogues and export them to foreign countries, recombinant bovine trypsin must be used.

The recombinant bovine trypsin independently developed and produced by our company has eliminated the hidden danger of animal virus, and provided a technically feasible scheme for the export of domestic third generation insulin to European and American countries.

[Product Description]

Dosage form: colorless clear liquid

Preservation buffer: 50mM HAC, 50mm cacl2100mM NaCl, pH3.0

Enzyme activity: 180-200 U / mg protein

Activity unit: under the condition of 25 ℃, pH 8.1, reaction system 3.0ml, enzyme hydrolysis of TAME per minute increased the absorption value at 247nm by 0.18, which was defined as 1 U unit.

General information:

Enzymology No.: EC3.4.21.4

Source: microbial recombinant protein

Molecular weight: 25kDa (SDS-PAGE detection)

Purity: > 90% (SDS-PAGE test)

Storage conditions: store at 4 ℃ for about one year (store at - 20 ℃ or - 80 ℃ after sub packaging according to the dosage for a longer time, avoid repeated freezing and thawing as much as possible)

Application: for insulin production