Description
TCEP HCl is a reducing agent suitable for protein applications. TCEP is often used for the reduction of disulfide bonds within proteins. This versatile compound is very soluble in aqueous solutions (310 grams/L in water). TCEP is stable in both acidic and basic solutions (pH 1.5 to 8.5).
TCEP HCL has many other advantages such as:
TCEP HCl is stable in air.
TCEP HCl is odorless eliminating the need to preform reactions within a fume hood.
TCEP HCl is efficient and can be used to reduce disulfide bonds at low concentrations (5 mM).
TCEP HCl may be used as a substitute for DTT (dithiothreitol) at a final concentration of 50 mM.
Suzhou Yacoo Science Corporation product TCEP HCl (Item No: S0178) has mature synthetic process with high quality. The purity of TCEP HCl can be made more than 98%. We can provide the products from grams to kilograms,Tris(2-carboxyethyl)phosphine hydrochloride is one of our most advantages diagnostic reagent products.
Specifications
Product Name: Tris(2-carboxyethyl)phosphine hydrochloride
Short Name：TCEP HCl
CAS No: 51805-45-9
Yacoo Item No: S0178
Formula: C9H16ClO6P
Molecular Weight: 286.647
Appearance: White crystal
Assay: ≥98.0%
Usage: TCEP-HCL is a pharmaceutical intermediates, chemical reagent; Reducing agent buffer
Storage：2-8℃
Packing: 25kg/Drum, or as your request
English Name：Tris(2-carboxyethyl)phosphine hydrochloride
Molecular Formula：C9H16ClO6P
Molecular Wt：286.65
CAS：51805-45-9
Shelf life: two years
Storage：2-8℃
|
SPECIFICATION PROPERTIES
|
STANDARD
|
|
Appearance
|
White crystal
|
|
Assay,%
|
≥98.0
|
|
MP,℃
|
170.0-180.0
|
What is the difference between DTT and TCEP-HCl in the reduction of disulfide bond in protein?
Disulfide bond (−S−S− bond) is a kind of covalent bond between S atoms. It plays an important role in the formation of three-dimensional structure of protein molecules. In order to identify the primary structure of protein, the disulfide bond must be opened firstly. DTT and TCEP-HCl are commonly used for the reduction of disulfide bond, then what is the difference between them?
Difference between DTT and TCEP
|
Prducts
|
DTT
|
TCEP
|
|
Smell
|
Slight sulfur smell
|
No
|
|
Soluble
|
50 g/L
|
310 g/L
|
|
reducibility
|
3-7 days
|
2-3 weeks
|
|
stability
|
Week volatility，in the presence of Ni2 it is rapid oxidized
|
Non-volatile,anti-air oxidation; not affected by metal ions.
|
|
pH
|
6.5-9.0
|
1.5-8.5
|
|
working concentration
|
1-100mM
|
5-50mM（most systems)
|
|
protein tagged with “His”
|
avoid
|
apply
|
|
Maleimide labeled protein reaction
|
Removed before marking
|
Non-removed before marking
|
