Sarcosine oxidase (SOX) CAS 18856-79-3

Sarcosine oxidase (EC. 1.5.3.1, SOX) belongs to flavin oxidase. It can catalyze the oxidation-reduction of N-methyl in sarcosine with fad as a cofactor. It is one of the key enzymes to detect the content of creatinine in serum or urine. It is widely used in the field of medical diagnosis.

Creatinine is produced under the catalysis of creatinine amide hydrolase, creatine is hydrolyzed to creatine and urea under the catalysis of creatine enzyme, creatine is then catalyzed by creatine oxidase to generate glycine, formaldehyde and hydrogen peroxide. Hydrogen peroxide, 2,4,6-triiodo-3-hydroxybenzoic acid and 4-aminoantipyrine react to form purplish red compound under the catalysis of peroxidase.. In the field of clinical diagnosis, the enzyme is often used in combination with creatinase and creatinase to complete the detection of creatinine.

[Product Description]

Dosage form: light yellow powder or solution

Source: microbial recombinant expression protein

Save buffer: Tris buffer, pH7.4

General information:

Enzymology No.: EC1.5.3.1

Molecular weight: about 43kDa (SDS-PAGE detection)

Purity: ≥ 90% (SDS-PAGE detection)

Isoelectric point: 5.80

pH stability: 6.5-9.0

Enzyme activity: 14U / mg protein

Storage conditions: store at 4 ℃ for about one year (store at - 20 ℃ or - 80 ℃ after sub packaging according to the dosage for a longer time, avoid repeated freezing and thawing as much as possible)

Transportation conditions: ice bag with low temperature

Application: the protein can be used in the development and production of enzymatic myoliver Kit

Safety note: this reagent is only used for research and development or production, and is strictly prohibited for human or animal experiments